Two New and Nontoxigenic Pseudo-nitzschia species (Bacillariophyceae) from Chinese Southeast Coastal Waters

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Two New and Nontoxigenic Pseudo-nitzschia species (Bacillariophyceae) from Chinese Southeast Coastal Waters. / Chen, Xiu Mei; Pang, Jin Xiu; Huang, Chun Xiu; Lundholm, Nina; Teng, Sing Tung; Li, Aifeng; Li, Yang.

In: Journal of Phycology, Vol. 57, No. 1, 2021, p. 335-344.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Chen, XM, Pang, JX, Huang, CX, Lundholm, N, Teng, ST, Li, A & Li, Y 2021, 'Two New and Nontoxigenic Pseudo-nitzschia species (Bacillariophyceae) from Chinese Southeast Coastal Waters', Journal of Phycology, vol. 57, no. 1, pp. 335-344. https://doi.org/10.1111/jpy.13101

APA

Chen, X. M., Pang, J. X., Huang, C. X., Lundholm, N., Teng, S. T., Li, A., & Li, Y. (2021). Two New and Nontoxigenic Pseudo-nitzschia species (Bacillariophyceae) from Chinese Southeast Coastal Waters. Journal of Phycology, 57(1), 335-344. https://doi.org/10.1111/jpy.13101

Vancouver

Chen XM, Pang JX, Huang CX, Lundholm N, Teng ST, Li A et al. Two New and Nontoxigenic Pseudo-nitzschia species (Bacillariophyceae) from Chinese Southeast Coastal Waters. Journal of Phycology. 2021;57(1):335-344. https://doi.org/10.1111/jpy.13101

Author

Chen, Xiu Mei ; Pang, Jin Xiu ; Huang, Chun Xiu ; Lundholm, Nina ; Teng, Sing Tung ; Li, Aifeng ; Li, Yang. / Two New and Nontoxigenic Pseudo-nitzschia species (Bacillariophyceae) from Chinese Southeast Coastal Waters. In: Journal of Phycology. 2021 ; Vol. 57, No. 1. pp. 335-344.

Bibtex

@article{b665bde7ba8144e5b4db6757b5e95019,
title = "Two New and Nontoxigenic Pseudo-nitzschia species (Bacillariophyceae) from Chinese Southeast Coastal Waters",
abstract = "To explore the species diversity and toxin profile of Pseudo-nitzschia, monoclonal strains were established from Chinese southeast coastal waters. The morphology was examined under light and transmission electron microscopy. The internal transcribed spacer region of ribosomal DNA was sequenced for phylogenetic analyses, and the secondary structure of ITS2 was predicted and compared among allied taxa. A combination of morphological and molecular data showed the presence of two new species, Pseudo-nitzschia hainanensis sp. nov. and Pseudo-nitzschia taiwanensis sp. nov. Pseudo-nitzschia hainanensis was characterized by a dumpy-lanceolate valve with slightly blunt apices and a central nodule, as well as striae comprising two rows of poroids. Pseudo-nitzschia taiwanensis was characterized by a slender-lanceolate valve, and striae comprising one row of split poroids. The poroid structure mainly comprised two sectors. Both taxa constituted their own monophyletic lineage in the phylogenetic analyses inferred from ITS2 rDNA and were well differentiated from other Pseudo-nitzschia species. Morphologically, P. hainanensis and P. taiwanensis could be assigned to the Pseudo-nitzschia delicatissima and the Pseudo-nitzschia pseudodelicatissima complex, respectively. Particulate domoic acid was measured using liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS), but no detectable pDA was found. With the description of the two new species, the species diversity of genus Pseudo-nitzschia reaches 58 worldwide, among which 31 have been recorded from Chinese coastal waters.",
keywords = "Chinese coastal waters, morphology, phylogeny, Pseudo&#8208, nitzschia hainanensis, nitzschia taiwanensis, SP-NOV BACILLARIOPHYCEAE, DOMOIC ACID, DIATOM, STRAIT, ITS2, RNA, PHYLOGENY, DIVERSITY, SHELLFISH, TOXICITY",
author = "Chen, {Xiu Mei} and Pang, {Jin Xiu} and Huang, {Chun Xiu} and Nina Lundholm and Teng, {Sing Tung} and Aifeng Li and Yang Li",
year = "2021",
doi = "10.1111/jpy.13101",
language = "English",
volume = "57",
pages = "335--344",
journal = "Journal of Phycology",
issn = "0022-3646",
publisher = "Wiley-Blackwell",
number = "1",

}

RIS

TY - JOUR

T1 - Two New and Nontoxigenic Pseudo-nitzschia species (Bacillariophyceae) from Chinese Southeast Coastal Waters

AU - Chen, Xiu Mei

AU - Pang, Jin Xiu

AU - Huang, Chun Xiu

AU - Lundholm, Nina

AU - Teng, Sing Tung

AU - Li, Aifeng

AU - Li, Yang

PY - 2021

Y1 - 2021

N2 - To explore the species diversity and toxin profile of Pseudo-nitzschia, monoclonal strains were established from Chinese southeast coastal waters. The morphology was examined under light and transmission electron microscopy. The internal transcribed spacer region of ribosomal DNA was sequenced for phylogenetic analyses, and the secondary structure of ITS2 was predicted and compared among allied taxa. A combination of morphological and molecular data showed the presence of two new species, Pseudo-nitzschia hainanensis sp. nov. and Pseudo-nitzschia taiwanensis sp. nov. Pseudo-nitzschia hainanensis was characterized by a dumpy-lanceolate valve with slightly blunt apices and a central nodule, as well as striae comprising two rows of poroids. Pseudo-nitzschia taiwanensis was characterized by a slender-lanceolate valve, and striae comprising one row of split poroids. The poroid structure mainly comprised two sectors. Both taxa constituted their own monophyletic lineage in the phylogenetic analyses inferred from ITS2 rDNA and were well differentiated from other Pseudo-nitzschia species. Morphologically, P. hainanensis and P. taiwanensis could be assigned to the Pseudo-nitzschia delicatissima and the Pseudo-nitzschia pseudodelicatissima complex, respectively. Particulate domoic acid was measured using liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS), but no detectable pDA was found. With the description of the two new species, the species diversity of genus Pseudo-nitzschia reaches 58 worldwide, among which 31 have been recorded from Chinese coastal waters.

AB - To explore the species diversity and toxin profile of Pseudo-nitzschia, monoclonal strains were established from Chinese southeast coastal waters. The morphology was examined under light and transmission electron microscopy. The internal transcribed spacer region of ribosomal DNA was sequenced for phylogenetic analyses, and the secondary structure of ITS2 was predicted and compared among allied taxa. A combination of morphological and molecular data showed the presence of two new species, Pseudo-nitzschia hainanensis sp. nov. and Pseudo-nitzschia taiwanensis sp. nov. Pseudo-nitzschia hainanensis was characterized by a dumpy-lanceolate valve with slightly blunt apices and a central nodule, as well as striae comprising two rows of poroids. Pseudo-nitzschia taiwanensis was characterized by a slender-lanceolate valve, and striae comprising one row of split poroids. The poroid structure mainly comprised two sectors. Both taxa constituted their own monophyletic lineage in the phylogenetic analyses inferred from ITS2 rDNA and were well differentiated from other Pseudo-nitzschia species. Morphologically, P. hainanensis and P. taiwanensis could be assigned to the Pseudo-nitzschia delicatissima and the Pseudo-nitzschia pseudodelicatissima complex, respectively. Particulate domoic acid was measured using liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS), but no detectable pDA was found. With the description of the two new species, the species diversity of genus Pseudo-nitzschia reaches 58 worldwide, among which 31 have been recorded from Chinese coastal waters.

KW - Chinese coastal waters

KW - morphology

KW - phylogeny

KW - Pseudo&#8208

KW - nitzschia hainanensis

KW - nitzschia taiwanensis

KW - SP-NOV BACILLARIOPHYCEAE

KW - DOMOIC ACID

KW - DIATOM

KW - STRAIT

KW - ITS2

KW - RNA

KW - PHYLOGENY

KW - DIVERSITY

KW - SHELLFISH

KW - TOXICITY

U2 - 10.1111/jpy.13101

DO - 10.1111/jpy.13101

M3 - Journal article

C2 - 33174223

VL - 57

SP - 335

EP - 344

JO - Journal of Phycology

JF - Journal of Phycology

SN - 0022-3646

IS - 1

ER -

ID: 254997533