Two New and Nontoxigenic Pseudo-nitzschia species (Bacillariophyceae) from Chinese Southeast Coastal Waters

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  • Xiu Mei Chen
  • Jin Xiu Pang
  • Chun Xiu Huang
  • Lundholm, Nina
  • Sing Tung Teng
  • Aifeng Li
  • Yang Li

To explore the species diversity and toxin profile of Pseudo-nitzschia, monoclonal strains were established from Chinese southeast coastal waters. The morphology was examined under light and transmission electron microscopy. The internal transcribed spacer region of ribosomal DNA was sequenced for phylogenetic analyses, and the secondary structure of ITS2 was predicted and compared among allied taxa. A combination of morphological and molecular data showed the presence of two new species, Pseudo-nitzschia hainanensis sp. nov. and Pseudo-nitzschia taiwanensis sp. nov. Pseudo-nitzschia hainanensis was characterized by a dumpy-lanceolate valve with slightly blunt apices and a central nodule, as well as striae comprising two rows of poroids. Pseudo-nitzschia taiwanensis was characterized by a slender-lanceolate valve, and striae comprising one row of split poroids. The poroid structure mainly comprised two sectors. Both taxa constituted their own monophyletic lineage in the phylogenetic analyses inferred from ITS2 rDNA and were well differentiated from other Pseudo-nitzschia species. Morphologically, P. hainanensis and P. taiwanensis could be assigned to the Pseudo-nitzschia delicatissima and the Pseudo-nitzschia pseudodelicatissima complex, respectively. Particulate domoic acid was measured using liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS), but no detectable pDA was found. With the description of the two new species, the species diversity of genus Pseudo-nitzschia reaches 58 worldwide, among which 31 have been recorded from Chinese coastal waters.

Original languageEnglish
JournalJournal of Phycology
Issue number1
Pages (from-to)335-344
Number of pages10
Publication statusPublished - 2021

    Research areas

  • Chinese coastal waters, morphology, phylogeny, Pseudo&#8208, nitzschia hainanensis, nitzschia taiwanensis, SP-NOV BACILLARIOPHYCEAE, DOMOIC ACID, DIATOM, STRAIT, ITS2, RNA, PHYLOGENY, DIVERSITY, SHELLFISH, TOXICITY

ID: 254997533