Monitoring of environmental DNA from nonindigenous species of algae, dinoflagellates and animals in the North East Atlantic
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Monitoring of environmental DNA from nonindigenous species of algae, dinoflagellates and animals in the North East Atlantic. / Knudsen, Steen Wilhelm; Hesselsøe, Martin; Thaulow, Jens; Agersnap, Sune; Hansen, Brian Klitgaard; Jacobsen, Magnus Wulff; Bekkevold, Dorte; Jensen, Søren K. S.; Møller, Peter Rask; Andersen, Jesper H.
I: Science of the Total Environment, Bind 821, 153093, 2022.Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt
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TY - JOUR
T1 - Monitoring of environmental DNA from nonindigenous species of algae, dinoflagellates and animals in the North East Atlantic
AU - Knudsen, Steen Wilhelm
AU - Hesselsøe, Martin
AU - Thaulow, Jens
AU - Agersnap, Sune
AU - Hansen, Brian Klitgaard
AU - Jacobsen, Magnus Wulff
AU - Bekkevold, Dorte
AU - Jensen, Søren K. S.
AU - Møller, Peter Rask
AU - Andersen, Jesper H.
N1 - Publisher Copyright: © 2022 The Authors
PY - 2022
Y1 - 2022
N2 - Monitoring the distribution of marine nonindigenous species is a challenging task. To support this monitoring, we developed and validated the specificity of 12 primer-probe assays for detection of environmental DNA (eDNA) from marine species, all nonindigenous to Europe. The species include sturgeons, a Pacific red algae, oyster thief, a freshwater hydroid from the Black Sea, Chinese mitten crab, Pacific oyster, warty comb jelly, sand gaper, round goby, pink salmon, rainbow trout and North American mud crab. We tested all assays in the laboratory, on DNA extracted from both the target and non-target species to ensure that they only amplified DNA from the intended species. Subsequently, all assays were used to analyse water samples collected at 16 different harbours across two different seasons during 2017. We also included six previously published assays targeting eDNA from goldfish, European carp, two species of dinoflagellates of the genera Karenia and Prorocentrum, two species of the heterokont flagellate genus Pseudochattonella. Conventional monitoring was carried out alongside eDNA sampling but with only one sampling event over the one year. Because eDNA was relatively fast and easy to collect compared to conventional sampling, we sampled eDNA twice during 2017, which showed seasonal changes in the distribution of nonindigenous species. Comparing eDNA levels with salinity gradients did not show any correlation. A significant correlation was observed between number of species detected with conventional monitoring methods and number of species found using eDNA at each location. This supports the use of eDNA for surveillance of the distribution of marine nonindigenous species, where the speed and relative easy sampling in the field combined with fast molecular analysis may provide advantages compared to conventional monitoring methods. Prior validation of assays increases taxonomic precision, and laboratorial setup facilitates analysis of multiple samples simultaneously. The specific eDNA assays presented here can be implemented directly in monitoring programmes across Europe and potentially worldwide to infer a more precise picture of the dynamics in the distribution of marine nonindigenous species.
AB - Monitoring the distribution of marine nonindigenous species is a challenging task. To support this monitoring, we developed and validated the specificity of 12 primer-probe assays for detection of environmental DNA (eDNA) from marine species, all nonindigenous to Europe. The species include sturgeons, a Pacific red algae, oyster thief, a freshwater hydroid from the Black Sea, Chinese mitten crab, Pacific oyster, warty comb jelly, sand gaper, round goby, pink salmon, rainbow trout and North American mud crab. We tested all assays in the laboratory, on DNA extracted from both the target and non-target species to ensure that they only amplified DNA from the intended species. Subsequently, all assays were used to analyse water samples collected at 16 different harbours across two different seasons during 2017. We also included six previously published assays targeting eDNA from goldfish, European carp, two species of dinoflagellates of the genera Karenia and Prorocentrum, two species of the heterokont flagellate genus Pseudochattonella. Conventional monitoring was carried out alongside eDNA sampling but with only one sampling event over the one year. Because eDNA was relatively fast and easy to collect compared to conventional sampling, we sampled eDNA twice during 2017, which showed seasonal changes in the distribution of nonindigenous species. Comparing eDNA levels with salinity gradients did not show any correlation. A significant correlation was observed between number of species detected with conventional monitoring methods and number of species found using eDNA at each location. This supports the use of eDNA for surveillance of the distribution of marine nonindigenous species, where the speed and relative easy sampling in the field combined with fast molecular analysis may provide advantages compared to conventional monitoring methods. Prior validation of assays increases taxonomic precision, and laboratorial setup facilitates analysis of multiple samples simultaneously. The specific eDNA assays presented here can be implemented directly in monitoring programmes across Europe and potentially worldwide to infer a more precise picture of the dynamics in the distribution of marine nonindigenous species.
KW - eDNA
KW - Introduced species
KW - Monitoring and assessment
KW - Non-indigenous species
KW - North Eastern Atlantic Ocean
KW - North Sea-Baltic Sea transition zone
KW - Quantitative PCR
KW - Real-time polymerase chain reaction
KW - Species-specific assays
U2 - 10.1016/j.scitotenv.2022.153093
DO - 10.1016/j.scitotenv.2022.153093
M3 - Journal article
C2 - 35038516
AN - SCOPUS:85123713650
VL - 821
JO - Science of the Total Environment
JF - Science of the Total Environment
SN - 0048-9697
M1 - 153093
ER -
ID: 309120487