TY - JOUR

T1 - Towards a new classification of Muscidae (Diptera)

T2 - a comparison of hypotheses based on multiple molecular phylogenetic approaches

AU - Grzywacz, Andrzej

AU - Trzeciak, Paulina

AU - Wiegmann, Brian M.

AU - Cassel, Brian K.

AU - Pape, Thomas

AU - Walczak, Kinga

AU - Bystrowski, Cezary

AU - Nelson, Leanne

AU - Piwczynski, Marcin

PY - 2021

Y1 - 2021

N2 - Muscidae are a megadiverse dipteran family that exhibits extraordinary diversity in morphology and life history as both immatures and adults. The classification of Muscidae has been long debated, and most higher-level relationships remain unknown. In this study, we used multilocus Sanger sequencing (mS-seq), anchored hybrid enrichment (AHE) and restriction-site associated DNA sequencing (RAD-seq) approaches to examine relationships within Muscidae. The results from AHE and RAD-seq largely correspond to those obtained from mS-seq in terms of overall topology, yet phylogenomic approaches received much higher nodal support. The results from all molecular approaches contradict the traditional classification based predominantly on adult morphology, but provide an opportunity to re-interpret the morphology of immature stages. Rearrangements in Muscidae classification are proposed as follows: (i) Mesembrina Meigen and Polietes Rondani are transferred from Muscinae to Azeliinae; (ii) Reinwardtiinae stat. rev. is resurrected as a subfamily distinct from Azeliinae; (iii) Eginia Robineau-Desvoidy, Neohelina Malloch, Syngamoptera Schnabl and Xenotachina Malloch are transferred to Reinwardtiinae stat. rev.

AB - Muscidae are a megadiverse dipteran family that exhibits extraordinary diversity in morphology and life history as both immatures and adults. The classification of Muscidae has been long debated, and most higher-level relationships remain unknown. In this study, we used multilocus Sanger sequencing (mS-seq), anchored hybrid enrichment (AHE) and restriction-site associated DNA sequencing (RAD-seq) approaches to examine relationships within Muscidae. The results from AHE and RAD-seq largely correspond to those obtained from mS-seq in terms of overall topology, yet phylogenomic approaches received much higher nodal support. The results from all molecular approaches contradict the traditional classification based predominantly on adult morphology, but provide an opportunity to re-interpret the morphology of immature stages. Rearrangements in Muscidae classification are proposed as follows: (i) Mesembrina Meigen and Polietes Rondani are transferred from Muscinae to Azeliinae; (ii) Reinwardtiinae stat. rev. is resurrected as a subfamily distinct from Azeliinae; (iii) Eginia Robineau-Desvoidy, Neohelina Malloch, Syngamoptera Schnabl and Xenotachina Malloch are transferred to Reinwardtiinae stat. rev.

KW - 3RD INSTAR LARVAE

KW - SEQUENCE ALIGNMENTS

KW - FORENSIC IMPORTANCE

KW - SPECIES-TREE

KW - MISSING DATA

KW - HOUSE-FLY

KW - MORPHOLOGY

KW - INFERENCE

KW - GENOME

KW - PHYLOGENOMICS

U2 - 10.1111/syen.12473

DO - 10.1111/syen.12473

M3 - Journal article

VL - 46

SP - 508

EP - 525

JO - Systematic Entomology

JF - Systematic Entomology

SN - 0307-6970

IS - 3

ER -