Occurrence of Pseudo-nitzschia species and associated domoic acid production along the Guangdong coast, South China Sea
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Occurrence of Pseudo-nitzschia species and associated domoic acid production along the Guangdong coast, South China Sea. / Dong, Huan Chang; Lundholm, Nina; Teng, Sing Tung; Li, Aifeng; Wang, Chao; Hu, Yang; Li, Yang.
In: Harmful Algae, Vol. 98, 101899, 2020.Research output: Contribution to journal › Journal article › Research › peer-review
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T1 - Occurrence of Pseudo-nitzschia species and associated domoic acid production along the Guangdong coast, South China Sea
AU - Dong, Huan Chang
AU - Lundholm, Nina
AU - Teng, Sing Tung
AU - Li, Aifeng
AU - Wang, Chao
AU - Hu, Yang
AU - Li, Yang
PY - 2020
Y1 - 2020
N2 - The diatom genus Pseudo-nitzschia, which has been associated with amnesic shellfish poisoning events globally, is also one of the key harmful microalga groups in Guangdong coastal waters, off the north coast of the South China Sea. In order to explore the diversity and toxigenic characteristics, Pseudo-nitzschia isolates were established. Based on a combination of morphological and molecular features, in total 26 different Pseudo-nitzschia taxa were identified, including two new species, P. uniseriata H.C. Dong & Yang Li and P. yuensis H.C. Dong & Yang Li. Morphologically, P. uniseriata is unique by having striae mainly comprising one row of poroids, which are simple without divided hymen internally, and each poroid containing one, seldom two sectors. Pseudonitzschia yuensis is characterized by having striae comprising one to two rows of poroids. In biseriate striae, the poroids are polygonal and irregularly distributed, and a discontinuous row of poroids may be present in the middle. In uniseriate striae, the poroids usually contain 1-5 sectors. Both taxa are well differentiated from other Pseudo-nitzschia species in phylogenetic analyses inferred from ITS2 sequence-structure information. Pseudonitzschia uniseriata is sister to P. lineola, whereas P. yuensis forms a group together with P. micropora and P. delicatissima. When comparing ITS2 secondary structure, two hemi-compensatory base change (HCBCs) are found between P. uniseriata and P. lineola. One compensatory base change (CBC) and four HCBCs are found between P. yuensis and P. delicatissima, and there is one CBC and five HCBCs between P. yuensis and P. micropora. The ability of cultured strains to produce particulate DA (pDA) revealed production of pDA in twenty-nine strains belonging to seven species: P. bipertita, P. caciantha, P. cuspidata, P. fraudulenta, P. fukuyoi, P. lundholmiae and P. multiseries. This is the first report of P. bipertita being toxic, with pDA content of 15.6 +/- 2.1 fg cell(-1). The presence of brine shrimps significantly increased pDA content in P. cuspidata, P. fukuyoi, P. lundholmiae and P. multiseries 1.4 to 7 times, and induced pDA production in P. fraudulenta from below detection limit to 17.5 +/- 1.6 fg cell(-1). The highest pDA concentration, 4830.5 +/- 120.3 fg cell(-1), was detected in P. multiseries, a level much lower than previous reports on P. multiseries from North America and Europe. Overall, the cellular toxin levels in Pseudo-nitzschia spp. were low in Guangdong coastal isolates.
AB - The diatom genus Pseudo-nitzschia, which has been associated with amnesic shellfish poisoning events globally, is also one of the key harmful microalga groups in Guangdong coastal waters, off the north coast of the South China Sea. In order to explore the diversity and toxigenic characteristics, Pseudo-nitzschia isolates were established. Based on a combination of morphological and molecular features, in total 26 different Pseudo-nitzschia taxa were identified, including two new species, P. uniseriata H.C. Dong & Yang Li and P. yuensis H.C. Dong & Yang Li. Morphologically, P. uniseriata is unique by having striae mainly comprising one row of poroids, which are simple without divided hymen internally, and each poroid containing one, seldom two sectors. Pseudonitzschia yuensis is characterized by having striae comprising one to two rows of poroids. In biseriate striae, the poroids are polygonal and irregularly distributed, and a discontinuous row of poroids may be present in the middle. In uniseriate striae, the poroids usually contain 1-5 sectors. Both taxa are well differentiated from other Pseudo-nitzschia species in phylogenetic analyses inferred from ITS2 sequence-structure information. Pseudonitzschia uniseriata is sister to P. lineola, whereas P. yuensis forms a group together with P. micropora and P. delicatissima. When comparing ITS2 secondary structure, two hemi-compensatory base change (HCBCs) are found between P. uniseriata and P. lineola. One compensatory base change (CBC) and four HCBCs are found between P. yuensis and P. delicatissima, and there is one CBC and five HCBCs between P. yuensis and P. micropora. The ability of cultured strains to produce particulate DA (pDA) revealed production of pDA in twenty-nine strains belonging to seven species: P. bipertita, P. caciantha, P. cuspidata, P. fraudulenta, P. fukuyoi, P. lundholmiae and P. multiseries. This is the first report of P. bipertita being toxic, with pDA content of 15.6 +/- 2.1 fg cell(-1). The presence of brine shrimps significantly increased pDA content in P. cuspidata, P. fukuyoi, P. lundholmiae and P. multiseries 1.4 to 7 times, and induced pDA production in P. fraudulenta from below detection limit to 17.5 +/- 1.6 fg cell(-1). The highest pDA concentration, 4830.5 +/- 120.3 fg cell(-1), was detected in P. multiseries, a level much lower than previous reports on P. multiseries from North America and Europe. Overall, the cellular toxin levels in Pseudo-nitzschia spp. were low in Guangdong coastal isolates.
KW - South China Sea
KW - Guangdong coast
KW - Pseudo-nitzschia uniseriata
KW - Pseudo-nitzschia yuensis
KW - Morphology
KW - Phylogeny
KW - Secondary structure
KW - Domoic acid
KW - SP-NOV BACILLARIOPHYCEAE
KW - COMPLEX BACILLARIOPHYCEAE
KW - MALAYSIAN BORNEO
KW - NAVIS-VARINGICA
KW - ALGAL TOXINS
KW - H. PERAGALLO
KW - DIATOM
KW - DIVERSITY
KW - PHYLOGENY
KW - MORPHOLOGY
U2 - 10.1016/j.hal.2020.101899
DO - 10.1016/j.hal.2020.101899
M3 - Journal article
C2 - 33129456
VL - 98
JO - Harmful Algae
JF - Harmful Algae
SN - 1568-9883
M1 - 101899
ER -
ID: 251636681