Cytogenetics in the sacoglossan Oxynoe olivacea (Mollusca: Opisthobranchia): Karyotype, chromosome banding nd fluorescent in situ hybridization
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Cytogenetics in the sacoglossan Oxynoe olivacea (Mollusca : Opisthobranchia): Karyotype, chromosome banding nd fluorescent in situ hybridization. / Vitturi, R.; Gianguzza, P.; Colomba, M. S.; Jensen, K. R.; Riggio, S.
In: Marine Biology, Vol. 137, No. 4, 2000, p. 577-582.Research output: Contribution to journal › Journal article › Research › peer-review
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TY - JOUR
T1 - Cytogenetics in the sacoglossan Oxynoe olivacea (Mollusca
T2 - Opisthobranchia): Karyotype, chromosome banding nd fluorescent in situ hybridization
AU - Vitturi, R.
AU - Gianguzza, P.
AU - Colomba, M. S.
AU - Jensen, K. R.
AU - Riggio, S.
PY - 2000
Y1 - 2000
N2 - Developing embryos and sexually mature follicles of the male portion of ovotestis proved to be a suitable material as a source of cleaving cells for advanced cytological investigations on the sacoglossan species Oxynoe olivacea Rafinesque, 1819 (Mollusca: Opisthobranchia). O. olivacea has a diploid chromosomal number of 30 made up of 15 pairs of which six are metacentric/submetacentric (M/SM), four subtelocentric (ST) and five on the borderline between SM and ST. Correspondingly, 15 bivalents occur in spermatocytes at Metaphase I. Constitutive heterochromatin is scarce and restricted to small C-bands seen in five pachytene bivalents. The use of combined silver staining and fluorescent in situ hybridization (FISH). with a Paracentrotus lividus (Echinodermata) 4.3 kilobase (kb) rDNA probe (prR14) consisting of sequences from the 3' end of 18S rDNA to the 3' end of 26S rDNA, revealed that nucleolus organizer regions (NORs) are situated terminally on one arm of a small metacentric pair. The telomeric (TTAGGG)(n) sequence did not hybridize with termini of O. olivacea chromosomes.
AB - Developing embryos and sexually mature follicles of the male portion of ovotestis proved to be a suitable material as a source of cleaving cells for advanced cytological investigations on the sacoglossan species Oxynoe olivacea Rafinesque, 1819 (Mollusca: Opisthobranchia). O. olivacea has a diploid chromosomal number of 30 made up of 15 pairs of which six are metacentric/submetacentric (M/SM), four subtelocentric (ST) and five on the borderline between SM and ST. Correspondingly, 15 bivalents occur in spermatocytes at Metaphase I. Constitutive heterochromatin is scarce and restricted to small C-bands seen in five pachytene bivalents. The use of combined silver staining and fluorescent in situ hybridization (FISH). with a Paracentrotus lividus (Echinodermata) 4.3 kilobase (kb) rDNA probe (prR14) consisting of sequences from the 3' end of 18S rDNA to the 3' end of 26S rDNA, revealed that nucleolus organizer regions (NORs) are situated terminally on one arm of a small metacentric pair. The telomeric (TTAGGG)(n) sequence did not hybridize with termini of O. olivacea chromosomes.
UR - http://www.scopus.com/inward/record.url?scp=0033724817&partnerID=8YFLogxK
U2 - 10.1007/s002270000377
DO - 10.1007/s002270000377
M3 - Journal article
AN - SCOPUS:0033724817
VL - 137
SP - 577
EP - 582
JO - Marine Biology
JF - Marine Biology
SN - 0025-3162
IS - 4
ER -
ID: 346073216