TY - JOUR

T1 - A Study of Chaetoceros debilis Sensu Lato Species (Bacillariophyceae), with Ememdation of C. debilis and Description of C. galeatus Sp. Nov.

AU - Xu, Xiaojing

AU - Lundholm, Nina

AU - Li, Yang

PY - 2020

Y1 - 2020

N2 - Chaetoceros debilisis considered one of the most abundant and widespread diatoms in the coastal marine phytoplankton, and is often used in research studies ranging from ecophysiology and molecular biology to oceanography and aquaculture. To clarify the species delineation ofC. debilisand explore the diversity amongC. debilissensu lato taxa, monoclonal strains were established from different geographical regions, including the Danish coast, close to the type locality ofC. debilis, the Denmark Strait (between Iceland and Greenland) as well as the Taiwan Strait, East China Sea and Daya Bay, South China Sea. Vegetative cells and resting spores were examined using light and electron microscopy. The hypervariable D1-D3 region of the nuclear large subunit ribosomal gene and the small subunit ribosomal gene were sequenced to address phylogenetic relationships. In bothSSUandLSUtrees, theC. debilissensu lato strains clustered in four distinct clades. Culture material from the type locality was, along with molecular data, used to delineate and emend the description ofC. debilis. Based on molecular data and detailed morphological features, one of the clades originating from Chinese waters was described asC. galeatussp. nov., characterized by curved and helical chains, elliptical valves, and smooth and helmet-shaped primary valves of the resting spores. The remaining two clades, which also represent two novel taxa,C. cf.debilis-1 andC. cf.debilis-2, were not formally described as new species due to the insufficient information on their morphology.

AB - Chaetoceros debilisis considered one of the most abundant and widespread diatoms in the coastal marine phytoplankton, and is often used in research studies ranging from ecophysiology and molecular biology to oceanography and aquaculture. To clarify the species delineation ofC. debilisand explore the diversity amongC. debilissensu lato taxa, monoclonal strains were established from different geographical regions, including the Danish coast, close to the type locality ofC. debilis, the Denmark Strait (between Iceland and Greenland) as well as the Taiwan Strait, East China Sea and Daya Bay, South China Sea. Vegetative cells and resting spores were examined using light and electron microscopy. The hypervariable D1-D3 region of the nuclear large subunit ribosomal gene and the small subunit ribosomal gene were sequenced to address phylogenetic relationships. In bothSSUandLSUtrees, theC. debilissensu lato strains clustered in four distinct clades. Culture material from the type locality was, along with molecular data, used to delineate and emend the description ofC. debilis. Based on molecular data and detailed morphological features, one of the clades originating from Chinese waters was described asC. galeatussp. nov., characterized by curved and helical chains, elliptical valves, and smooth and helmet-shaped primary valves of the resting spores. The remaining two clades, which also represent two novel taxa,C. cf.debilis-1 andC. cf.debilis-2, were not formally described as new species due to the insufficient information on their morphology.

KW - Chaetoceros debilis

KW - Chaetoceros galeatus

KW - morphology

KW - phylogeny

KW - resting spore

KW - GENUS CHAETOCEROS

KW - IDENTIFICATION

KW - DIVERSITY

KW - DIATOM

KW - CRITERIA

KW - SEA

U2 - 10.1111/jpy.12982

DO - 10.1111/jpy.12982

M3 - Journal article

C2 - 32110841

VL - 56

SP - 784

EP - 797

JO - Journal of Phycology

JF - Journal of Phycology

SN - 0022-3646

IS - 3

ER -