Using DNA Metabarcoding to Identify Floral Visitation by Pollinators

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Standard

Using DNA Metabarcoding to Identify Floral Visitation by Pollinators. / Lowe, Abigail; Jones, Laura; Witter, Lucy; Creer, Simon; de Vere, Natasha.

I: Diversity, Bind 14, Nr. 4, 236, 2022.

Publikation: Bidrag til tidsskrift › Tidsskriftartikel › Forskning › fagfællebedømt

Harvard

Lowe, A, Jones, L, Witter, L, Creer, S & de Vere, N 2022, 'Using DNA Metabarcoding to Identify Floral Visitation by Pollinators', Diversity, bind 14, nr. 4, 236. https://doi.org/10.3390/d14040236

APA

Lowe, A., Jones, L., Witter, L., Creer, S., & de Vere, N. (2022). Using DNA Metabarcoding to Identify Floral Visitation by Pollinators. Diversity, 14(4), [236]. https://doi.org/10.3390/d14040236

Vancouver

Lowe A, Jones L, Witter L, Creer S, de Vere N. Using DNA Metabarcoding to Identify Floral Visitation by Pollinators. Diversity. 2022;14(4). 236. https://doi.org/10.3390/d14040236

Author

Lowe, Abigail ; Jones, Laura ; Witter, Lucy ; Creer, Simon ; de Vere, Natasha. / Using DNA Metabarcoding to Identify Floral Visitation by Pollinators. I: Diversity. 2022 ; Bind 14, Nr. 4.

Bibtex

@article{36d6ac71a00c40c6ac604902a118e1c1,
title = "Using DNA Metabarcoding to Identify Floral Visitation by Pollinators",
abstract = "The identification of floral visitation by pollinators provides an opportunity to improve our understanding of the fine-scale ecological interactions between plants and pollinators, contributing to biodiversity conservation and promoting ecosystem health. In this review, we outline the various methods which can be used to identify floral visitation, including plant-focused and insect-focused methods. We reviewed the literature covering the ways in which DNA metabarcoding has been used to answer ecological questions relating to plant use by pollinators and discuss the findings of this research. We present detailed methodological considerations for each step of the metabarcoding workflow, from sampling through to amplification, and finally bioinformatic analysis. Detailed guidance is provided to researchers for utilisation of these techniques, emphasising the importance of standardisation of methods and improving the reliability of results. Future opportunities and directions of using molecular methods to analyse plant–pollinator interactions are then discussed.",
author = "Abigail Lowe and Laura Jones and Lucy Witter and Simon Creer and {de Vere}, Natasha",
year = "2022",
doi = "10.3390/d14040236",
language = "English",
volume = "14",
journal = "Diversity",
issn = "1424-2818",
publisher = "M D P I AG",
number = "4",

}

RIS

TY - JOUR

T1 - Using DNA Metabarcoding to Identify Floral Visitation by Pollinators

AU - Lowe, Abigail

AU - Jones, Laura

AU - Witter, Lucy

AU - Creer, Simon

AU - de Vere, Natasha

PY - 2022

Y1 - 2022

N2 - The identification of floral visitation by pollinators provides an opportunity to improve our understanding of the fine-scale ecological interactions between plants and pollinators, contributing to biodiversity conservation and promoting ecosystem health. In this review, we outline the various methods which can be used to identify floral visitation, including plant-focused and insect-focused methods. We reviewed the literature covering the ways in which DNA metabarcoding has been used to answer ecological questions relating to plant use by pollinators and discuss the findings of this research. We present detailed methodological considerations for each step of the metabarcoding workflow, from sampling through to amplification, and finally bioinformatic analysis. Detailed guidance is provided to researchers for utilisation of these techniques, emphasising the importance of standardisation of methods and improving the reliability of results. Future opportunities and directions of using molecular methods to analyse plant–pollinator interactions are then discussed.

AB - The identification of floral visitation by pollinators provides an opportunity to improve our understanding of the fine-scale ecological interactions between plants and pollinators, contributing to biodiversity conservation and promoting ecosystem health. In this review, we outline the various methods which can be used to identify floral visitation, including plant-focused and insect-focused methods. We reviewed the literature covering the ways in which DNA metabarcoding has been used to answer ecological questions relating to plant use by pollinators and discuss the findings of this research. We present detailed methodological considerations for each step of the metabarcoding workflow, from sampling through to amplification, and finally bioinformatic analysis. Detailed guidance is provided to researchers for utilisation of these techniques, emphasising the importance of standardisation of methods and improving the reliability of results. Future opportunities and directions of using molecular methods to analyse plant–pollinator interactions are then discussed.

U2 - 10.3390/d14040236

DO - 10.3390/d14040236

M3 - Journal article

VL - 14

JO - Diversity

JF - Diversity

SN - 1424-2818

IS - 4

M1 - 236

ER -

ID: 302403070