This report is the outcome of MONIS 2 – or in full, “Monitoring of Non-Indigenous Species in Danish Marine Water, phase 2” – and includes three deliverable: (1) a national Target Species List including 50 species, (2) a draft Technical Guidance Report, and (3) in silico designed and tested primers and probes for 48 of the 50 species on the Target Species List. The list is based on discussions at a workshop and subsequent scoring and ranking of relevant species. The draft Technical Guidance report is anchored in existing Standard Operating Procedures (i.e. protocols for sampling, storage and analysis) and adapted to the requirements of the Danish NOVANA programme. In addition, the report includes suggestions for next steps to take to implement and improve monitoring and assessment activities in regard to non-indigenous species in Danish marine waters.