TY - JOUR

T1 - From DNA to biomass

T2 - Opportunities and challenges in species quantification of bulk fisheries products

AU - Hansen, Brian Klitgaard

AU - Farrant, Gregory Kevin

AU - Ogden, Rob

AU - Humble, Emily

AU - Ólafsdóttir, Guðbjörg

AU - Bekkevold, Dorte

AU - Knudsen, Steen Wilhelm

AU - Møller, Peter Rask

AU - Nielsen, Einar Eg

N1 - Publisher Copyright: © 2020 Oxford University Press. All rights reserved.

PY - 2020

Y1 - 2020

N2 - Fisheries enforcement relies on visual catch identification and quantification at sea or when landed. Silage (fish dissolved in acid) and fish blocks (block frozen fish) are promising methods for on-board processing and storage of low-value catches. We examined the use of nondestructive sampling and two DNA-based methods, quantitative PCR (qPCR) and metabarcoding, to assess species composition and relative abundance in industrial grade experimental silage and fish blocks. We demonstrate the ability to identify and quantify DNA from fish species in both products. qPCR analysis of small silage samples collected over 21 days detected all target control species. DNA from one species (Atlantic wolffish) was consistently overrepresented while, for three species of gadoids (Atlantic cod, haddock and whiting), the DNA content matched input tissue proportions with high accuracy. qPCR and metabarcoding of fish blocks, sampled as run-off water and exterior swabs, provided consistent species detection, with the highest variance observed in quantification from swab samples. Our analysis shows that DNA-based methods have significant potential as a tool for species identification and quantification of complex on-board-processed seafood products and are readily applicable to taxonomically and morphologically similar fish. There is, however, a need for establishing DNA/weight calibration factors for primary fisheries species.

AB - Fisheries enforcement relies on visual catch identification and quantification at sea or when landed. Silage (fish dissolved in acid) and fish blocks (block frozen fish) are promising methods for on-board processing and storage of low-value catches. We examined the use of nondestructive sampling and two DNA-based methods, quantitative PCR (qPCR) and metabarcoding, to assess species composition and relative abundance in industrial grade experimental silage and fish blocks. We demonstrate the ability to identify and quantify DNA from fish species in both products. qPCR analysis of small silage samples collected over 21 days detected all target control species. DNA from one species (Atlantic wolffish) was consistently overrepresented while, for three species of gadoids (Atlantic cod, haddock and whiting), the DNA content matched input tissue proportions with high accuracy. qPCR and metabarcoding of fish blocks, sampled as run-off water and exterior swabs, provided consistent species detection, with the highest variance observed in quantification from swab samples. Our analysis shows that DNA-based methods have significant potential as a tool for species identification and quantification of complex on-board-processed seafood products and are readily applicable to taxonomically and morphologically similar fish. There is, however, a need for establishing DNA/weight calibration factors for primary fisheries species.

KW - DNA quantification

KW - metabarcoding

KW - MinION

KW - multi-species fisheries products

KW - quantitative PCR

U2 - 10.1093/icesjms/fsaa115

DO - 10.1093/icesjms/fsaa115

M3 - Journal article

AN - SCOPUS:85100332745

VL - 77

SP - 2557

EP - 2566

JO - ICES Journal of Marine Science

JF - ICES Journal of Marine Science

SN - 1054-3139

IS - 7-8

ER -