TY - JOUR

T1 - A guide to avian museomics

T2 - Insights gained from resequencing hundreds of avian study skins

AU - Irestedt, Martin

AU - Thörn, Filip

AU - Müller, Ingo A.

AU - Jønsson, Knud A.

AU - Ericson, Per G. P.

AU - Blom, Mozes P. K.

PY - 2022

Y1 - 2022

N2 - Biological specimens in natural history collections constitute a massive repository of genetic information. Many specimens have been collected in areas in which they no longer exist or in areas where present-day collecting is not possible. There are also specimens in collections representing populations or species that have gone extinct. Furthermore, species or populations may have been sampled throughout an extensive time period, which is particularly valuable for studies of genetic change through time. With the advent of high-throughput sequencing, natural history museum resources have become accessible for genomic research. Consequently, these unique resources are increasingly being used across many fields of natural history. In this paper, we summarize our experiences of resequencing hundreds of genomes from historical avian museum specimens. We publish the protocols we have used and discuss the entire workflow from sampling and laboratory procedures, to the bioinformatic processing of historical specimen data.

AB - Biological specimens in natural history collections constitute a massive repository of genetic information. Many specimens have been collected in areas in which they no longer exist or in areas where present-day collecting is not possible. There are also specimens in collections representing populations or species that have gone extinct. Furthermore, species or populations may have been sampled throughout an extensive time period, which is particularly valuable for studies of genetic change through time. With the advent of high-throughput sequencing, natural history museum resources have become accessible for genomic research. Consequently, these unique resources are increasingly being used across many fields of natural history. In this paper, we summarize our experiences of resequencing hundreds of genomes from historical avian museum specimens. We publish the protocols we have used and discuss the entire workflow from sampling and laboratory procedures, to the bioinformatic processing of historical specimen data.

KW - birds

KW - genomic libraries

KW - museomics

KW - natural history collections

KW - ANCIENT DNA

KW - MITOCHONDRIAL-DNA

KW - MUSEUM SPECIMENS

KW - SEQUENCES

KW - COLLECTIONS

KW - RADIATION

KW - PARADISE

KW - REVEALS

KW - HISTORY

KW - FORMAT

U2 - 10.1111/1755-0998.13660

DO - 10.1111/1755-0998.13660

M3 - Journal article

C2 - 35661418

VL - 22

SP - 2672

EP - 2684

JO - Molecular Ecology

JF - Molecular Ecology

SN - 0962-1083

IS - 7

ER -