DNA barcoding a taxonomically complex hemiparasitic genus reveals deep divergence between ploidy levels but lack of species-level resolution

Research output: Contribution to journal › Journal article › Research › peer-review

Standard

DNA barcoding a taxonomically complex hemiparasitic genus reveals deep divergence between ploidy levels but lack of species-level resolution. / Wang, Xumei; Gussarova, Galina; Ruhsam, Markus; de Vere, Natasha; Metherell, Chris; Hollingsworth, Peter M.; Twyford, Alex D.

In: A O B Plants, Vol. 10, No. 3, ply026, 2018.

Research output: Contribution to journal › Journal article › Research › peer-review

Harvard

Wang, X, Gussarova, G, Ruhsam, M, de Vere, N, Metherell, C, Hollingsworth, PM & Twyford, AD 2018, 'DNA barcoding a taxonomically complex hemiparasitic genus reveals deep divergence between ploidy levels but lack of species-level resolution', A O B Plants, vol. 10, no. 3, ply026. https://doi.org/10.1093/aobpla/ply026

APA

Wang, X., Gussarova, G., Ruhsam, M., de Vere, N., Metherell, C., Hollingsworth, P. M., & Twyford, A. D. (2018). DNA barcoding a taxonomically complex hemiparasitic genus reveals deep divergence between ploidy levels but lack of species-level resolution. A O B Plants, 10(3), [ply026]. https://doi.org/10.1093/aobpla/ply026

Vancouver

Wang X, Gussarova G, Ruhsam M, de Vere N, Metherell C, Hollingsworth PM et al. DNA barcoding a taxonomically complex hemiparasitic genus reveals deep divergence between ploidy levels but lack of species-level resolution. A O B Plants. 2018;10(3). ply026. https://doi.org/10.1093/aobpla/ply026

Author

Wang, Xumei ; Gussarova, Galina ; Ruhsam, Markus ; de Vere, Natasha ; Metherell, Chris ; Hollingsworth, Peter M. ; Twyford, Alex D. / DNA barcoding a taxonomically complex hemiparasitic genus reveals deep divergence between ploidy levels but lack of species-level resolution. In: A O B Plants. 2018 ; Vol. 10, No. 3.

Bibtex

@article{c73896e7130a43a3a7f4eb5697303576,
title = "DNA barcoding a taxonomically complex hemiparasitic genus reveals deep divergence between ploidy levels but lack of species-level resolution",
abstract = "DNA barcoding is emerging as a useful tool not only for species identification but also for studying evolutionary and ecological processes. Although plant DNA barcodes do not always provide species-level resolution, the generation of large DNA barcode data sets can provide insights into the mechanisms underlying the generation of species diversity. Here, we study evolutionary processes in taxonomically complex British Euphrasia (Orobanchaceae), a group with multiple ploidy levels, frequent self-fertilization, young species divergence and widespread hybridization. We use a phylogenetic approach to investigate the colonization history of British Euphrasia, followed by a DNA barcoding survey and population genetic analyses to reveal the causes of shared sequence variation. Phylogenetic analysis shows Euphrasia have colonized Britain from mainland Europe on multiple occasions. DNA barcoding reveals that no British Euphrasia species has a consistent diagnostic sequence profile, and instead, plastid haplotypes are either widespread across species, or are population specific. The partitioning of nuclear genetic variation suggests differences in ploidy act as a barrier to gene exchange, while the divergence between diploid and tetraploid ITS sequences supports the polyploids being allotetraploid in origin. Overall, these results show that even when lacking species-level resolution, analyses of DNA barcoding data can reveal evolutionary patterns in taxonomically complex genera.",
author = "Xumei Wang and Galina Gussarova and Markus Ruhsam and {de Vere}, Natasha and Chris Metherell and Hollingsworth, {Peter M.} and Twyford, {Alex D.}",
year = "2018",
doi = "10.1093/aobpla/ply026",
language = "English",
volume = "10",
journal = "AoB PLANTS",
issn = "2041-2851",
publisher = "Oxford University Press",
number = "3",

}

RIS

TY - JOUR

T1 - DNA barcoding a taxonomically complex hemiparasitic genus reveals deep divergence between ploidy levels but lack of species-level resolution

AU - Wang, Xumei

AU - Gussarova, Galina

AU - Ruhsam, Markus

AU - de Vere, Natasha

AU - Metherell, Chris

AU - Hollingsworth, Peter M.

AU - Twyford, Alex D.

PY - 2018

Y1 - 2018

N2 - DNA barcoding is emerging as a useful tool not only for species identification but also for studying evolutionary and ecological processes. Although plant DNA barcodes do not always provide species-level resolution, the generation of large DNA barcode data sets can provide insights into the mechanisms underlying the generation of species diversity. Here, we study evolutionary processes in taxonomically complex British Euphrasia (Orobanchaceae), a group with multiple ploidy levels, frequent self-fertilization, young species divergence and widespread hybridization. We use a phylogenetic approach to investigate the colonization history of British Euphrasia, followed by a DNA barcoding survey and population genetic analyses to reveal the causes of shared sequence variation. Phylogenetic analysis shows Euphrasia have colonized Britain from mainland Europe on multiple occasions. DNA barcoding reveals that no British Euphrasia species has a consistent diagnostic sequence profile, and instead, plastid haplotypes are either widespread across species, or are population specific. The partitioning of nuclear genetic variation suggests differences in ploidy act as a barrier to gene exchange, while the divergence between diploid and tetraploid ITS sequences supports the polyploids being allotetraploid in origin. Overall, these results show that even when lacking species-level resolution, analyses of DNA barcoding data can reveal evolutionary patterns in taxonomically complex genera.

AB - DNA barcoding is emerging as a useful tool not only for species identification but also for studying evolutionary and ecological processes. Although plant DNA barcodes do not always provide species-level resolution, the generation of large DNA barcode data sets can provide insights into the mechanisms underlying the generation of species diversity. Here, we study evolutionary processes in taxonomically complex British Euphrasia (Orobanchaceae), a group with multiple ploidy levels, frequent self-fertilization, young species divergence and widespread hybridization. We use a phylogenetic approach to investigate the colonization history of British Euphrasia, followed by a DNA barcoding survey and population genetic analyses to reveal the causes of shared sequence variation. Phylogenetic analysis shows Euphrasia have colonized Britain from mainland Europe on multiple occasions. DNA barcoding reveals that no British Euphrasia species has a consistent diagnostic sequence profile, and instead, plastid haplotypes are either widespread across species, or are population specific. The partitioning of nuclear genetic variation suggests differences in ploidy act as a barrier to gene exchange, while the divergence between diploid and tetraploid ITS sequences supports the polyploids being allotetraploid in origin. Overall, these results show that even when lacking species-level resolution, analyses of DNA barcoding data can reveal evolutionary patterns in taxonomically complex genera.

U2 - 10.1093/aobpla/ply026

DO - 10.1093/aobpla/ply026

M3 - Journal article

VL - 10

JO - AoB PLANTS

JF - AoB PLANTS

SN - 2041-2851

IS - 3

M1 - ply026

ER -

ID: 284973689