TY - CHAP

T1 - Carbohydrate microarrays in plant science

AU - Fangel, Jonatan Ulrik

AU - Pedersen, Henriette Lodberg

AU - Vidal Melgosa, Silvia

AU - Ahl, Louise Isager

AU - Asunción Salmeán, Armando

AU - Madsen, Jack Egelund

AU - Rydahl, Maja Gro

AU - Clausen, Mads Hartvig

AU - Willats, William George Tycho

PY - 2012

Y1 - 2012

N2 - Almost all plant cells are surrounded by glycan-rich cell walls, which form much of the plant body and collectively are the largest source of biomass on earth. Plants use polysaccharides for support, defense, signaling, cell adhesion, and as energy storage, and many plant glycans are also important industrially and nutritionally. Understanding the biological roles of plant glycans and the effective exploitation of their useful properties requires a detailed understanding of their structures, occurrence, and molecular interactions. Microarray technology has revolutionized the massively high-throughput analysis of nucleotides, proteins, and increasingly carbohydrates. Using microarrays, the abundance of and interactions between hundreds and thousands of molecules can be assessed simultaneously using very small amounts of analytes. Here we show that carbohydrate microarrays are multifunctional tools for plant research and can be used to map glycan populations across large numbers of samples to screen antibodies, carbohydrate binding proteins, and carbohydrate binding modules and to investigate enzyme activities.

AB - Almost all plant cells are surrounded by glycan-rich cell walls, which form much of the plant body and collectively are the largest source of biomass on earth. Plants use polysaccharides for support, defense, signaling, cell adhesion, and as energy storage, and many plant glycans are also important industrially and nutritionally. Understanding the biological roles of plant glycans and the effective exploitation of their useful properties requires a detailed understanding of their structures, occurrence, and molecular interactions. Microarray technology has revolutionized the massively high-throughput analysis of nucleotides, proteins, and increasingly carbohydrates. Using microarrays, the abundance of and interactions between hundreds and thousands of molecules can be assessed simultaneously using very small amounts of analytes. Here we show that carbohydrate microarrays are multifunctional tools for plant research and can be used to map glycan populations across large numbers of samples to screen antibodies, carbohydrate binding proteins, and carbohydrate binding modules and to investigate enzyme activities.

KW - Carbohydrates

KW - Microarray Analysis

KW - Oligosaccharides

KW - Plants

U2 - 10.1007/978-1-61779-995-2_19

DO - 10.1007/978-1-61779-995-2_19

M3 - Book chapter

C2 - 22893299

SN - 978-1-61779-994-5

T3 - Methods in Molecular Biology

SP - 351

EP - 362

BT - High-throughput phenotyping in plants

A2 - Normanly, Jennifer

PB - Springer

ER -