A guide to avian museomics: Insights gained from resequencing hundreds of avian study skins

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Biological specimens in natural history collections constitute a massive repository of genetic information. Many specimens have been collected in areas in which they no longer exist or in areas where present-day collecting is not possible. There are also specimens in collections representing populations or species that have gone extinct. Furthermore, species or populations may have been sampled throughout an extensive time period, which is particularly valuable for studies of genetic change through time. With the advent of high-throughput sequencing, natural history museum resources have become accessible for genomic research. Consequently, these unique resources are increasingly being used across many fields of natural history. In this paper, we summarize our experiences of resequencing hundreds of genomes from historical avian museum specimens. We publish the protocols we have used and discuss the entire workflow from sampling and laboratory procedures, to the bioinformatic processing of historical specimen data.

Original languageEnglish
JournalMolecular Ecology Resources
Volume22
Issue number7
Pages (from-to)2672-2684
Number of pages13
ISSN1755-098X
DOIs
Publication statusPublished - 2022

    Research areas

  • birds, genomic libraries, museomics, natural history collections, ANCIENT DNA, MITOCHONDRIAL-DNA, MUSEUM SPECIMENS, SEQUENCES, COLLECTIONS, RADIATION, PARADISE, REVEALS, HISTORY, FORMAT

ID: 312338294